Identification of mono- or poly-specific monoclonal antibody to Porphyromonas gingivalis heat-shock protein 60
최점일, 이상률, 김관회, 최봉규, 김명진,
소속 상세정보
최점일 ( Choi Jeom-Il ) - Pusan National University College of Dentistry Department of Periodontology
이상률 ( Lee Sang-Yull ) - Pusan National University School of Medicine Department of Biochemistry
김관회 ( Kim Koan-Hoi ) - Pusan National University School of Medicine Department of Pharmacology
최봉규 ( Choi Bong-Kyu ) - Seoul National University School of Dentistry Department of Microbiology and Immunology
김명진 ( Kim Myung-Jin ) - Pusan National University College of Dentistry Department of Periodontology
KMID : 0363020110410020054
Abstract
Purpose: The aim of this study was to define the immunoreactive specificity of Porphyromonas gingivalis (P. gingivalis) heat shock protein (HSP) 60 in periodontitis and atherosclerosis.
Methods: In an attempt to define the cross-reactive bacterial heat-shock protein with human self-antigen at molecular level, we have introduced a novel strategy for cloning hybridoma producing anti-P. gingivalis HSP 60 which is polyreactive to bacterial HSPs or to the human homolog.
Results: Five cross-reactive clones were obtained which recognized the #19 peptide (TLVVNRLRGSLKICAVKAPG) among 37 synthetic peptides (20-mer, 5 amino acids overlapping) spanning the whole molecule of P. gingivalis HSP 60. We have also established three anti-P. gingivalis HSP 60 monoclonal antibodies demonstrating mono-specificity. These clones recognized the #29 peptide (TVPGGGTTYIRAIAALEGLK).
Conclusions: Peptide #19 and #29 of P. gingivalis HSP 60 might be important immunoreactive epitopes in the immunopathogenic mechanism of bacterial antigen-triggered autoimmune diseases.
키워드
Antibodies; Periodontitis; Porphyromonas gingivalis
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